Hotspot

rrn16S

IRb · 101828-103318 · 1491 bp

Back to Disporum

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rrn16S

Feature Metadata

label_name: rrn16S
feature_type: rRNA
region: IRb
length_bp: 1491
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16S_p1	CGAATCCGCTTTGTCTACGA	CAGACGTAGCTCGCTTCTTT	50.0	50.0	58.1	58.0	1622 bp	1.0	79.1
rrn16S_p2	CCAGGCTAATATGAAGCGCA	CAGACGTAGCTCGCTTCTTT	50.0	50.0	58.1	58.0	1678 bp	1.0	78.9
rrn16S_p3	TCCAGGCTAATATGAAGCGC	CAGACGTAGCTCGCTTCTTT	50.0	50.0	57.8	58.0	1679 bp	1.0	78.5
rrn16S_p4	CGAATCCGCTTTGTCTACGA	TGTGAAGCAGTGTCAAACCA	50.0	45.0	58.1	57.9	1598 bp	1.0	78.3
rrn16S_p5	CCAGGCTAATATGAAGCGCA	TGTGAAGCAGTGTCAAACCA	50.0	45.0	58.1	57.9	1654 bp	1.0	78.1