Hotspot

rbcL

LSC · 55791-57236 · 1446 bp

Back to Disporum

Pi 0.00911

MarkerSeek score 45.50

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1446
variable_sites: 22
pi: 0.00911
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGAGTTGTAGGGAGGGACTT	GGGCCGAGTTTAATTGCAAC	50.0	50.0	57.3	58.0	1525-1550 bp	1.0	75.3
rbcL_p2	TGAGTTGTAGGGAGGGACTT	AAGGAAAAGATTGGGCCGAG	50.0	50.0	57.3	58.2	1537-1562 bp	1.0	74.1
rbcL_p3	TCATGAGTTGTAGGGAGGGA	GGGCCGAGTTTAATTGCAAC	50.0	50.0	57.1	58.0	1528-1553 bp	1.0	73.5
rbcL_p4	CATGAGTTGTAGGGAGGGAC	GGGCCGAGTTTAATTGCAAC	55.0	50.0	57.0	58.0	1527-1552 bp	1.0	73.2
rbcL_p5	TGAGTTGTAGGGAGGGACTT	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.3	58.4	1526-1551 bp	1.0	72.4