Hotspot

ndhG-ndhI

SSC · 120524-120931 · 408 bp

Back to Disporum

Pi 0.00449

MarkerSeek score 57.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 408
variable_sites: 3
pi: 0.00449
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TACCCCGAGACCCCCTAATA	ACTCAAATCAACATAGACGGGG	55.0	45.5	58.5	58.1	552-553 bp	1.0	59.8
ndhG-ndhI_p2	TACCCCGAGACCCCCTAATA	CTCAAATCAACATAGACGGGGA	55.0	45.5	58.5	57.9	551-552 bp	1.0	59.7
ndhG-ndhI_p3	TACCCCGAGACCCCCTAATA	TCAAATCAACATAGACGGGGA	55.0	42.9	58.5	56.7	550-551 bp	1.0	58.6
ndhG-ndhI_p4	TAATACTACCCCGAGACCCC	ACTCAAATCAACATAGACGGGG	55.0	45.5	57.3	58.1	558-559 bp	1.0	58.0
ndhG-ndhI_p5	TAATACTACCCCGAGACCCC	CTCAAATCAACATAGACGGGGA	55.0	45.5	57.3	57.9	557-558 bp	1.0	57.9