Hotspot

rpl32-trnL-UAG

SSC · 114897-115705 · 809 bp

Back to Disporum

Pi 0.013

MarkerSeek score 61.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 809
variable_sites: 20
pi: 0.013
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CAGTTTCCACCGGACATTCA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	902-905 bp	1.0	80.9
rpl32-trnL-UAG_p2	CAGTTTCCACCGGACATTCA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	903-906 bp	1.0	80.9
rpl32-trnL-UAG_p3	TCAGTTTCCACCGGACATTC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.8	58.0	903-906 bp	1.0	80.4
rpl32-trnL-UAG_p4	TCAGTTTCCACCGGACATTC	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.8	58.0	904-907 bp	1.0	80.3
rpl32-trnL-UAG_p5	CAGTTTCCACCGGACATTCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	912-915 bp	1.0	80.0