Hotspot

ndhF-rpl32

SSC · 113923-114722 · 800 bp

Back to Disporum

Pi 0.0098

MarkerSeek score 42.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 800
variable_sites: 14
pi: 0.0098
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ATACGTGAAGGGGGAATTGC	TGAATGTCCGGTGGAAACTG	50.0	50.0	57.9	58.1	1671-1674 bp	1.0	79.8
ndhF-rpl32_p2	TTGCGCGGATTTAGCAATTG	TGAATGTCCGGTGGAAACTG	45.0	50.0	58.1	58.1	1655-1658 bp	1.0	79.5
ndhF-rpl32_p3	GGCACACAGGGTAGCAAATA	TGAATGTCCGGTGGAAACTG	50.0	50.0	57.9	58.1	1616-1619 bp	1.0	79.3
ndhF-rpl32_p4	ATACGTGAAGGGGGAATTGC	GAATGTCCGGTGGAAACTGA	50.0	50.0	57.9	57.8	1670-1673 bp	1.0	79.2
ndhF-rpl32_p5	GGATCGATCAAGTGTCCGAG	TGAATGTCCGGTGGAAACTG	55.0	50.0	57.9	58.1	1201 bp	0.25	48.1