Hotspot

ndhC-trnV-UAC

LSC · 50734-52022 · 1289 bp

Back to Disporum

Pi 0.0104

MarkerSeek score 56.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1289
variable_sites: 23
pi: 0.0104
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGGTGCTAAAACTCCGGAAA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	57.1	57.8	1415-1433 bp	1.0	72.6
ndhC-trnV-UAC_p2	AGGTGCTAAAACTCCGGAAA	GAAGGTCTACGGTTCGAGTC	45.0	55.0	57.1	57.5	1414-1432 bp	1.0	69.8
ndhC-trnV-UAC_p3	AGGTGCTAAAACTCCGGAAA	GTTCGAGTCCGTATAGCCCT	45.0	55.0	57.1	58.7	1403-1421 bp	1.0	68.7
ndhC-trnV-UAC_p4	AGGTGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	42.9	50.0	57.6	57.8	1415-1433 bp	1.0	68.6
ndhC-trnV-UAC_p5	AGGTGCTAAAACTCCGGAAA	AAGGTCTACGGTTCGAGTCC	45.0	55.0	57.1	58.8	1413-1431 bp	1.0	67.5