Hotspot

trnL-UAA-trnF-GAA

LSC · 47914-48337 · 424 bp

Back to Disporum

Pi 0.00829

MarkerSeek score 57.90

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 424
variable_sites: 6
pi: 0.00829
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	TACGTGTCAATACCGACAGC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.7	58.1	563-574 bp	1.0	79.8
trnL-UAA-trnF-GAA_p2	TACGTGTCAATACCGACAGC	CACGTGTCAGGAACCAGATT	50.0	50.0	57.7	57.8	581-592 bp	1.0	78.8
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	503-514 bp	1.0	78.5
trnL-UAA-trnF-GAA_p4	TACGTGTCAATACCGACAGC	TAGTCACGTGTCAGGAACCA	50.0	50.0	57.7	58.3	585-596 bp	1.0	77.9
trnL-UAA-trnF-GAA_p5	CGTGTCAATACCGACAGCAA	TTTGAACTGGTGACACGAGG	50.0	50.0	58.6	58.1	561-572 bp	1.0	77.4