Hotspot

trnC-GCA-petN

LSC · 27958-29152 · 1195 bp

Back to Disporum

Pi 0.00777

MarkerSeek score 51.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1195
variable_sites: 17
pi: 0.00777
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TCATTTTGGGTCGGATTGGG	CCATTAAAACAGCCCAAGCG	50.0	50.0	58.2	58.0	1307-1321 bp	1.0	80.8
trnC-GCA-petN_p2	TCATTTTGGGTCGGATTGGG	AGTCCACTCCTTCCCCATAC	50.0	55.0	58.2	58.1	1356-1370 bp	1.0	79.8
trnC-GCA-petN_p3	TCATTTTGGGTCGGATTGGG	GTCCACTCCTTCCCCATACT	50.0	55.0	58.2	58.1	1355-1369 bp	1.0	79.8
trnC-GCA-petN_p4	TCATTTTGGGTCGGATTGGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.2	58.2	1297-1311 bp	1.0	79.6
trnC-GCA-petN_p5	TCATTTTGGGTCGGATTGGG	GAGTCCACTCCTTCCCCATA	50.0	55.0	58.2	57.8	1357-1371 bp	1.0	79.4