Hotspot

rpl16

LSC · 82341-83796 · 1456 bp

Back to Disporopsis

Pi 0.00252

MarkerSeek score 38.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1456
variable_sites: 6
pi: 0.00252
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACCCCTTTTGTTTATCTTTAGTTCT	TCCTTTTTCCGTTAAATCAAACA	32.0	30.4	56.6	55.3	1577-1590 bp	1.0	40.5
rpl16_p2	ACCCCTTTTGTTTATCTTTAGTTCT	TCCTTTTTCCGTTAAATCAAACAA	32.0	29.2	56.6	56.0	1577-1590 bp	1.0	40.5
rpl16_p3	ACCCCTTTTGTTTATCTTTAGTTCT	TTCCTTTTTCCGTTAAATCAAACA	32.0	29.2	56.6	56.0	1578-1591 bp	1.0	40.5
rpl16_p4	ACCCCTTTTGTTTATCTTTAGTTC	TCCTTTTTCCGTTAAATCAAACA	33.3	30.4	55.3	55.3	1577-1590 bp	1.0	40.5
rpl16_p5	ACCCCTTTTGTTTATCTTTAGT	TCCTTTTTCCGTTAAATCAAACA	31.8	30.4	53.2	55.3	1577-1590 bp	1.0	40.5