Hotspot

rbcL

LSC · 56483-57922 · 1440 bp

Back to Disporopsis

Pi 0.000694

MarkerSeek score 41.70

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1440
variable_sites: 2
pi: 0.000694
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGAGTTGTAGGGAGGGACTT	AAAAGGATTGGGCCGAGTTT	50.0	45.0	57.3	58.0	1552 bp	1.0	74.3
rbcL_p2	TGAGTTGTAGGGAGGGACTT	TGAATTATGCGCGCCTAGTT	50.0	45.0	57.3	58.1	1493 bp	1.0	74.0
rbcL_p3	TGAGTTGTAGGGAGGGACTT	TTACTGAATTATGCGCGCCT	50.0	45.0	57.3	58.1	1497 bp	1.0	74.0
rbcL_p4	TGAGTTGTAGGGAGGGACTT	ATGCGCGCCTAGTTCTTATC	50.0	50.0	57.3	58.2	1487 bp	1.0	72.7
rbcL_p5	TCATGAGTTGTAGGGAGGGA	AAAAGGATTGGGCCGAGTTT	50.0	45.0	57.1	58.0	1555 bp	1.0	72.5