Hotspot

ndhA

SSC · 121210-123382 · 2173 bp

Back to Disporopsis

Pi 0.00146

MarkerSeek score 58.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2173
variable_sites: 6
pi: 0.00146
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCGGTCACCATAGGGAACAT	ATTGGCCGATATCATGACGA	55.0	45.0	59.2	56.9	2354-2355 bp	1.0	61.9
ndhA_p2	CCGGTCACCATAGGGAACAT	TTGGCCGATATCATGACGAT	55.0	45.0	59.2	56.9	2353-2354 bp	1.0	61.9
ndhA_p3	CCGGTCACCATAGGGAACAT	AATTGGCCGATATCATGACGA	55.0	42.9	59.2	57.6	2355-2356 bp	1.0	59.7
ndhA_p4	CCGGTCACCATAGGGAACAT	TGGCCGATATCATGACGATAC	55.0	47.6	59.2	57.5	2352-2353 bp	1.0	58.9
ndhA_p5	CCGGTCACCATAGGGAACAT	TGGGAGAAGTTGATCGTTGA	55.0	45.0	59.2	56.5	2310-2311 bp	1.0	58.8