Hotspot

rpl32-trnL-UAG

SSC · 114671-115607 · 937 bp

Back to Disporopsis

Pi 0.00445
MarkerSeek score 54.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
937
variable_sites
8
pi
0.00445
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAAACAAATAAGA AGCGTGTCTACCGATTTCAC 35.0 50.0 53.8 58.0 973-1000 bp 1.0 49.1
rpl32-trnL-UAG_p2 TGTGCGACAAACAAATAAGA CAGCGTGTCTACCGATTTCA 35.0 50.0 53.8 58.0 974-1001 bp 1.0 49.1
rpl32-trnL-UAG_p3 TGTGCGACAAACAAATAAGA TTTACAGTATGCCGCCACTC 35.0 50.0 53.8 58.0 1033-1060 bp 1.0 48.9
rpl32-trnL-UAG_p4 TGTGCGACAAACAAATAAGA TCCTAAGAGCAGCGTGTCTA 35.0 50.0 53.8 57.9 983-1010 bp 1.0 48.1
rpl32-trnL-UAG_p5 TGTGCGACAAACAAATAAGAA AGCGTGTCTACCGATTTCAC 33.3 50.0 54.6 58.0 973-1000 bp 1.0 48.0