Hotspot

rrn16S-trnI-GAU

IRb · 103504-103804 · 301 bp

Back to Disporopsis

Pi 0.00498

MarkerSeek score 56.00

Primer available yes

Label rrn16S-trnI-GAU

Feature Metadata

label_name: rrn16S-trnI-GAU
feature_type: igs
region: IRb
length_bp: 301
variable_sites: 3
pi: 0.00498
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16S-trnI-GAU_p1	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	55.0	55.0	57.7	57.8	427 bp	1.0	76.9
rrn16S-trnI-GAU_p2	CAAGGTAGCCGTACTGGAAG	CGCTGATGAGCACATTGAAC	55.0	50.0	57.7	57.8	446 bp	1.0	76.8
rrn16S-trnI-GAU_p3	AGTGACTGGAGTGAAGTCGT	CTATCCATGTGGCTGAGAGC	50.0	55.0	58.3	57.8	449 bp	1.0	76.7
rrn16S-trnI-GAU_p4	AGTGACTGGAGTGAAGTCGT	CGCTGATGAGCACATTGAAC	50.0	50.0	58.3	57.8	468 bp	1.0	76.7
rrn16S-trnI-GAU_p5	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	55.0	50.0	57.7	58.2	395 bp	1.0	76.7