Hotspot

petG-trnW-CCA

LSC · 67338-67461 · 124 bp

Back to Disporopsis

Pi 0.00403

MarkerSeek score 59.90

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 1
pi: 0.00403
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAATACAGACGCGGTGATC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	228-234 bp	1.0	81.3
petG-trnW-CCA_p2	CAATACAGACGCGGTGATCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	227-233 bp	1.0	81.3
petG-trnW-CCA_p3	ACAATACAGACGCGGTGATC	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	192-198 bp	1.0	81.1
petG-trnW-CCA_p4	CAATACAGACGCGGTGATCA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	191-197 bp	1.0	81.1
petG-trnW-CCA_p5	CGGTGATCAGTTGGACCTTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	216-222 bp	1.0	80.6