Hotspot

ycf4-cemA

LSC · 61479-62251 · 773 bp

Back to Disporopsis

Pi 0.00367

MarkerSeek score 61.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 5
pi: 0.00367
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGACTCCGCGAGAAATTGAG	ATGAGAGAGAGACCCACCAG	50.0	55.0	58.0	57.8	925-931 bp	1.0	79.5
ycf4-cemA_p2	TGAGAATTTGACTCCGCGAG	ATGAGAGAGAGACCCACCAG	50.0	55.0	58.0	57.8	933-939 bp	1.0	79.5
ycf4-cemA_p3	TGCGCGTACCAATTGAAGTA	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.9	57.8	877-883 bp	1.0	78.9
ycf4-cemA_p4	CTTGCGCGTACCAATTGAAG	ATGAGAGAGAGACCCACCAG	50.0	55.0	58.1	57.8	879-885 bp	1.0	78.6
ycf4-cemA_p5	AAAAAGCTGCTGAATTGGCC	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.8	57.8	904-910 bp	1.0	78.1