Hotspot

accD-psaI

LSC · 60089-60464 · 376 bp

Back to Disporopsis

Pi 0.0151

MarkerSeek score 65.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 376
variable_sites: 9
pi: 0.0151
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCAATCGTACCGCGTAATCT	AAAGAAGCCATTGCAATCGC	50.0	45.0	57.9	58.0	539-571 bp	1.0	84.2
accD-psaI_p2	CCAATCGTACCGCGTAATCT	GCAGGAAATACTAGGCCCAC	50.0	55.0	57.9	58.0	521-553 bp	1.0	84.0
accD-psaI_p3	AGTTATTTCAGCTGCACGGT	AAAGAAGCCATTGCAATCGC	45.0	45.0	57.8	58.0	499-531 bp	1.0	83.9
accD-psaI_p4	AGTTATTTCAGCTGCACGGT	GCAGGAAATACTAGGCCCAC	45.0	55.0	57.8	58.0	481-513 bp	1.0	83.7
accD-psaI_p5	ATTTCAGCTGCACGGTTTCT	AAAGAAGCCATTGCAATCGC	45.0	45.0	58.4	58.0	495-527 bp	1.0	82.3