Hotspot

rbcL-accD

LSC · 57923-58597 · 675 bp

Back to Disporopsis

Pi 0.00593

MarkerSeek score 53.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 6
pi: 0.00593
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGCTGCTTGTGAAGTATGGA	CCGGTATGTCCAACAGCATT	50.0	50.0	58.3	58.2	851-853 bp	1.0	76.5
rbcL-accD_p2	CGCTGCTTGTGAAGTATGGA	CTTCCACCGGTATGTCCAAC	50.0	55.0	58.3	58.3	857-859 bp	1.0	76.3
rbcL-accD_p3	AAATGGAGCCCTGAACTAGC	CCGGTATGTCCAACAGCATT	50.0	50.0	57.6	58.2	871-873 bp	1.0	75.2
rbcL-accD_p4	AAATGGAGCCCTGAACTAGC	CTTCCACCGGTATGTCCAAC	50.0	55.0	57.6	58.3	877-879 bp	1.0	75.1
rbcL-accD_p5	TCGAGTTCGAACCAGTGGAT	CCGGTATGTCCAACAGCATT	50.0	50.0	58.7	58.2	819-821 bp	1.0	72.7