Hotspot

trnS-GCU-trnG-UCC

LSC · 8461-9770 · 1310 bp

Back to Disporopsis

Pi 0.00254
MarkerSeek score 52.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1310
variable_sites
6
pi
0.00254
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 GATCAGAGGGAACGGAGAGA ACGAATCGCACTTTTACCACT 55.0 42.9 57.9 58.2 1432-1444 bp 1.0 71.1
trnS-GCU-trnG-UCC_p2 AAGTGATCAGAGGGAACGGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.1 58.2 1436-1448 bp 1.0 71.1
trnS-GCU-trnG-UCC_p3 ATCAGAGGGAACGGAGAGAG ACGAATCGCACTTTTACCACT 55.0 42.9 57.9 58.2 1431-1443 bp 1.0 71.1
trnS-GCU-trnG-UCC_p4 GATCAGAGGGAACGGAGAGA ACGAATCGCACTTTTACCAC 55.0 45.0 57.9 56.8 1432-1444 bp 1.0 71.1
trnS-GCU-trnG-UCC_p5 AAGTGATCAGAGGGAACGGA ACGAATCGCACTTTTACCAC 50.0 45.0 58.1 56.8 1436-1448 bp 1.0 71.1