Hotspot

rpl6

Genome · 96939-97478 · 540 bp

Back to Discostella

Pi 0.0765

MarkerSeek score 81.30

Primer available yes

Label rpl6

Feature Metadata

label_name: rpl6
feature_type: gene
region: Genome
length_bp: 540
variable_sites: 60
pi: 0.0765
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl6_p1	CGTCGTAAACGTTGGTCACA	TCATTACGGCGGGATGTTTT	50.0	45.0	58.8	57.9	1527-1529 bp	1.0	79.3
rpl6_p2	CGTCGTAAACGTTGGTCACA	TTCATTACGGCGGGATGTTT	50.0	45.0	58.8	57.9	1528-1530 bp	1.0	79.2
rpl6_p3	CGTCGTAAACGTTGGTCACA	TTTCATTACGGCGGGATGTT	50.0	45.0	58.8	57.9	1529-1531 bp	1.0	79.2
rpl6_p4	CGTCGTAAACGTTGGTCACA	CCCCATAAGTCTGGAAGCAC	50.0	55.0	58.8	58.0	1316-1318 bp	0.666667	65.3
rpl6_p5	CGTCGTAAACGTTGGTCACA	AAATTGTAGGCCACCTGCTC	50.0	50.0	58.8	58.2	1444 bp	0.333333	45.6