Hotspot

ccsA

Genome · 110525-111502 · 978 bp

Back to Discostella

Pi 0.0723
MarkerSeek score 80.00
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
Genome
length_bp
978
variable_sites
104
pi
0.0723
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 GCTGTTTCGCAACGTTTACA GACCAGTGGATGAAGTTGGA 45.0 50.0 57.9 57.1 1618 bp 0.333333 45.7
ccsA_p2 GCTGTTTCGCAACGTTTACA AGACCAGTGGATGAAGTTGG 45.0 50.0 57.9 57.1 1619 bp 0.333333 45.6
ccsA_p3 GCAACGTTTACACCCAACAC GACCAGTGGATGAAGTTGGA 50.0 50.0 58.2 57.1 1610 bp 0.333333 45.1
ccsA_p4 GCAACGTTTACACCCAACAC AGACCAGTGGATGAAGTTGG 50.0 50.0 58.2 57.1 1611 bp 0.333333 45.0
ccsA_p5 CAACCATCCCAAGGAACCAT GACCAGTGGATGAAGTTGGA 50.0 50.0 57.8 57.1 1549 bp 0.333333 44.6
ccsA_p1 CTCCCCAGGTAGGACTTGAA GCTGTTTCGCAACGTTTACA 55.0 45.0 58.0 57.9 1966-1968 bp 1.0 87.0
ccsA_p2 CTCCCCAGGTAGGACTTGAA GCAACGTTTACACCCAACAC 55.0 50.0 58.0 58.2 1958-1960 bp 1.0 86.3
ccsA_p3 AGGACTTGAACCTACGACCA GCTGTTTCGCAACGTTTACA 50.0 45.0 58.0 57.9 1958 bp 0.333333 52.5
ccsA_p4 AGGACTTGAACCTACGACCA GCAACGTTTACACCCAACAC 50.0 50.0 58.0 58.2 1950 bp 0.333333 51.9
ccsA_p5 AGGACTTGAACCTACGACCA CAACCATCCCAAGGAACCAT 50.0 50.0 58.0 57.8 1889 bp 0.333333 51.4