Hotspot

ycf89-trnP-UGG

Genome · 128254-128959 · 706 bp

Back to Discostella

Pi 0.232

MarkerSeek score 77.70

Primer available yes

Label ycf89-trnP-UGG

Feature Metadata

label_name: ycf89-trnP-UGG
feature_type: igs
region: Genome
length_bp: 706
variable_sites: 175
pi: 0.232
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf89-trnP-UGG_p1	TGTGCTTGATTTGGGGGAAT	AGGATTTGAACCTACGGCAC	45.0	50.0	57.7	57.9	816-976 bp	0.666667	75.2
ycf89-trnP-UGG_p2	ACCCAATCTACGGCATCAAC	AGGATTTGAACCTACGGCAC	50.0	50.0	58.0	57.9	1315 bp	0.333333	52.2
ycf89-trnP-UGG_p3	TTGTGCTTGATTTGGGGGAA	AGGATTTGAACCTACGGCAC	45.0	50.0	57.9	57.9	977 bp	0.333333	51.7
ycf89-trnP-UGG_p4	TGGCATTCCAGGAACATCTG	AGGATTTGAACCTACGGCAC	50.0	50.0	57.9	57.9	884 bp	0.333333	51.4
ycf89-trnP-UGG_p5	ATTGTGCTTGATTTGGGGGA	AGGATTTGAACCTACGGCAC	45.0	50.0	57.7	57.9	978 bp	0.333333	49.9