Hotspot

rns-ycf89

Genome · 126800-127290 · 491 bp

Back to Discostella

Pi 0.226

MarkerSeek score 79.60

Primer available yes

Label rns-ycf89

Feature Metadata

label_name: rns-ycf89
feature_type: igs
region: Genome
length_bp: 491
variable_sites: 137
pi: 0.226
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns-ycf89_p1	CATTGCGGAAAATTCCCCAC	GTTGATGCCGTAGATTGGGT	50.0	50.0	58.0	58.0	1282 bp	0.333333	53.0
rns-ycf89_p2	TCTCGTACGACTTGCATGTG	GTTGATGCCGTAGATTGGGT	50.0	50.0	58.0	58.0	1000 bp	0.333333	52.8
rns-ycf89_p3	TATTGCTCCGTCAGGCTTTC	GTTGATGCCGTAGATTGGGT	50.0	50.0	58.0	58.0	1305 bp	0.333333	52.8
rns-ycf89_p4	CAACCCATAGGCAGTCTTCC	GTTGATGCCGTAGATTGGGT	55.0	50.0	58.0	58.0	1334 bp	0.333333	52.7
rns-ycf89_p5	CTTGCATCCTCCGTCTTACC	GTTGATGCCGTAGATTGGGT	55.0	50.0	58.1	58.0	1433 bp	0.333333	52.6