Hotspot

thiS-psb28

Genome · 84755-85042 · 288 bp

Back to Discostella

Pi 0.26

MarkerSeek score 77.00

Primer available yes

Label thiS-psb28

Feature Metadata

label_name: thiS-psb28
feature_type: igs
region: Genome
length_bp: 288
variable_sites: 97
pi: 0.26
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
thiS-psb28_p1	TCACAACCCACTGCCTTAAC	CTTTGACTCCGCCATTCGTA	50.0	50.0	58.0	58.0	1061-1139 bp	1.0	91.8
thiS-psb28_p2	TCACAACCCACTGCCTTAAC	TTTGACTCCGCCATTCGTAG	50.0	50.0	58.0	58.0	1060-1138 bp	1.0	91.8
thiS-psb28_p3	GTGTTGACCAGGTCTAGCAG	CTTTGACTCCGCCATTCGTA	55.0	50.0	57.9	58.0	1266-1320 bp	0.666667	77.9
thiS-psb28_p4	GTGTTGACCAGGTCTAGCAG	TTTGACTCCGCCATTCGTAG	55.0	50.0	57.9	58.0	1265-1319 bp	0.666667	77.9
thiS-psb28_p5	TCACAACCCACTGCCTTAAC	TGGAACGCTATGCCAAAAGT	50.0	45.0	58.0	58.1	673 bp	0.333333	52.4