Hotspot

trnP-UGG-ycf89

Genome · 64702-65407 · 706 bp

Back to Discostella

Pi 0.232

MarkerSeek score 77.70

Primer available yes

Label trnP-UGG-ycf89

Feature Metadata

label_name: trnP-UGG-ycf89
feature_type: igs
region: Genome
length_bp: 706
variable_sites: 175
pi: 0.232
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-ycf89_p1	TGGATGACATTTACGGCAGG	ACCCAATCTACGGCATCAAC	50.0	50.0	58.0	58.0	1536 bp	0.333333	52.7
trnP-UGG-ycf89_p2	TGGTTGGATTGGATGGGTTG	ACCCAATCTACGGCATCAAC	50.0	50.0	58.1	58.0	1830 bp	0.333333	52.5
trnP-UGG-ycf89_p3	TGGATGACATTTACGGCAGG	TTGTGCTTGATTTGGGGGAA	50.0	45.0	58.0	57.9	1198 bp	0.333333	52.3
trnP-UGG-ycf89_p4	AGGATTTGAACCTACGGCAC	ACCCAATCTACGGCATCAAC	50.0	50.0	57.9	58.0	1315 bp	0.333333	52.2
trnP-UGG-ycf89_p5	TGGTTGGATTGGATGGGTTG	TTGTGCTTGATTTGGGGGAA	50.0	45.0	58.1	57.9	1492 bp	0.333333	52.1