Hotspot

psaJ-trnP-UGG

Genome · 64492-64627 · 136 bp

Back to Discostella

Pi 0.168

MarkerSeek score 79.40

Primer available yes

Label psaJ-trnP-UGG

Feature Metadata

label_name: psaJ-trnP-UGG
feature_type: igs
region: Genome
length_bp: 136
variable_sites: 32
pi: 0.168
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-trnP-UGG_p1	TGGTTGGATTGGATGGGTTG	GGTAGAGTACCTGCTTTGGG	50.0	55.0	58.1	57.3	556-561 bp	1.0	82.5
psaJ-trnP-UGG_p2	TTGGATTGGATGGGTTGGAC	GGTAGAGTACCTGCTTTGGG	50.0	55.0	57.8	57.3	558 bp	0.666667	65.6
psaJ-trnP-UGG_p3	TGGATGACATTTACGGCAGG	GGTAGAGTACCTGCTTTGGG	50.0	55.0	58.0	57.3	267 bp	0.333333	47.5
psaJ-trnP-UGG_p4	ATGTAGTGAGTCTCCAGCGT	GGTAGAGTACCTGCTTTGGG	50.0	55.0	58.2	57.3	822 bp	0.333333	46.5
psaJ-trnP-UGG_p5	CTCCACCAGCTCTAGCCTTA	GGTAGAGTACCTGCTTTGGG	55.0	55.0	58.2	57.3	736 bp	0.333333	46.0