Hotspot

petA-chlI

Genome · 43978-44652 · 675 bp

Back to Discostella

Pi 0.3

MarkerSeek score 84.10

Primer available yes

Label petA-chlI

Feature Metadata

label_name: petA-chlI
feature_type: igs
region: Genome
length_bp: 675
variable_sites: 252
pi: 0.3
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-chlI_p1	GGTGGCTTTGGTCAAGAAGA	GCAAAAGCACATGCAGCATA	50.0	45.0	58.0	58.0	1005 bp	0.333333	53.2
petA-chlI_p2	GTAATCGTGGTCGTGGTCAA	GCAAAAGCACATGCAGCATA	50.0	45.0	57.9	58.0	1247 bp	0.333333	52.8
petA-chlI_p3	GGTGGCTTTGGTCAAGAAGA	TCCGGCTCGATTTGTATTGG	50.0	50.0	58.0	58.1	1345 bp	0.333333	52.8
petA-chlI_p4	TTGGTGCTGATGGTAAACCC	GCAAAAGCACATGCAGCATA	50.0	45.0	58.1	58.0	1523 bp	0.333333	52.7
petA-chlI_p5	GTTGTTGCACCTGATCCAGA	GCAAAAGCACATGCAGCATA	50.0	45.0	58.1	58.0	1314 bp	0.333333	52.5