Hotspot

rpoB-rps20

Genome · 38200-38598 · 399 bp

Back to Discostella

Pi 0.207

MarkerSeek score 80.00

Primer available yes

Label rpoB-rps20

Feature Metadata

label_name: rpoB-rps20
feature_type: igs
region: Genome
length_bp: 399
variable_sites: 113
pi: 0.207
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-rps20_p1	ATAAGCGGCTCTCAGTAGGT	CGTTGCGGATTATTTCGACG	50.0	50.0	57.9	58.0	1542 bp	0.333333	52.6
rpoB-rps20_p2	AAGCGGCTCTCAGTAGGTAA	CGTTGCGGATTATTTCGACG	50.0	50.0	58.2	58.0	1540 bp	0.333333	52.0
rpoB-rps20_p3	AGCGGCTCTCAGTAGGTAAA	CGTTGCGGATTATTTCGACG	50.0	50.0	58.2	58.0	1539 bp	0.333333	52.0
rpoB-rps20_p4	ATAAGCGGCTCTCAGTAGGT	GCAGCGCTACTAAAAACTGC	50.0	50.0	57.9	58.1	934 bp	0.333333	51.8
rpoB-rps20_p5	AGCGGCTCTCAGTAGGTAAA	GCAGCGCTACTAAAAACTGC	50.0	50.0	58.2	58.1	931 bp	0.333333	51.2