primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbX-rbcS_p1	psbX-rbcS	1	GCGATAAAATTTGCTAATGAAGTTGT	CCCTGAAGGTAGCCGTTATT	26	20	30.8	50.0	57.8	57.0	0.0	0.0	7.175778	7545	7966	408-476	1.000000	52.0
psbX-rbcS_p2	psbX-rbcS	2	GCGATAAAATTTGCTAATGAAGTTGT	CAGTGTTCAAGCTAACCCTGA	26	21	30.8	47.6	57.8	57.9	0.0	6.1	7.299322	7545	7966	423-491	1.000000	52.0
psbX-rbcS_p3	psbX-rbcS	3	GCGATAAAATTTGCTAATGAAGTTGT	TGTTCAAGCTAACCCTGAAGG	26	21	30.8	47.6	57.8	57.9	0.0	0.0	7.313646	7545	7966	420-488	1.000000	52.0
psbX-rbcS_p4	psbX-rbcS	4	GCGATAAAATTTGCTAATGAAGTTGT	ACAGTGTTCAAGCTAACCCTG	26	21	30.8	47.6	57.8	58.1	0.0	9.5	7.322126	7545	7966	424-492	1.000000	52.0
psbX-rbcS_p5	psbX-rbcS	5	GCGATAAAATTTGCTAATGAAGTTGT	ACAGTGTTCAAGCTAACCCT	26	20	30.8	45.0	57.8	56.7	0.0	0.0	7.498487	7545	7966	424-492	1.000000	52.0
rpl1-rpl12_p1	rpl1-rpl12	1	TGCGAAAATTATCCCGACGT	GCCATCATAATACCCCCACC	20	20	45.0	55.0	58.0	57.8	0.0	0.0	0.228879	26120	26199	903-909	0.666667	71.7
rpl1-rpl12_p2	rpl1-rpl12	2	TGCGAAAATTATCCCGACGT	AACGACGATGCTGTACGATC	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.189108	26120	26199	1359-1359	0.333333	51.8
rpl1-rpl12_p3	rpl1-rpl12	3	TGCGAAAATTATCCCGACGT	CGACGATGCTGTACGATCAA	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.190355	26120	26199	1357-1357	0.333333	51.8
rpl1-rpl12_p4	rpl1-rpl12	4	TGCGAAAATTATCCCGACGT	GGCCATCATAATACCCCCAC	20	20	45.0	55.0	58.0	57.8	0.0	0.0	0.228879	26120	26199	910-910	0.333333	51.5
rpl1-rpl12_p5	rpl1-rpl12	5	CAATTGCGTACAACGGTGAC	AACGACGATGCTGTACGATC	20	20	50.0	50.0	58.1	58.2	7.9	0.0	0.230964	26120	26199	1189-1189	0.333333	51.5
rpoB-rps20_p1	rpoB-rps20	1	ATAAGCGGCTCTCAGTAGGT	CGTTGCGGATTATTTCGACG	20	20	50.0	50.0	57.9	58.0	1.4	0.0	0.088204	38200	38598	1542-1542	0.333333	52.6
rpoB-rps20_p2	rpoB-rps20	2	AAGCGGCTCTCAGTAGGTAA	CGTTGCGGATTATTTCGACG	20	20	50.0	50.0	58.2	58.0	1.4	0.0	0.164823	38200	38598	1540-1540	0.333333	52.0
rpoB-rps20_p3	rpoB-rps20	3	AGCGGCTCTCAGTAGGTAAA	CGTTGCGGATTATTTCGACG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.164823	38200	38598	1539-1539	0.333333	52.0
rpoB-rps20_p4	rpoB-rps20	4	ATAAGCGGCTCTCAGTAGGT	GCAGCGCTACTAAAAACTGC	20	20	50.0	50.0	57.9	58.1	1.4	11.0	0.191253	38200	38598	934-934	0.333333	51.8
rpoB-rps20_p5	rpoB-rps20	5	AGCGGCTCTCAGTAGGTAAA	GCAGCGCTACTAAAAACTGC	20	20	50.0	50.0	58.2	58.1	0.0	11.0	0.267872	38200	38598	931-931	0.333333	51.2
petA-chlI_p1	petA-chlI	1	GGTGGCTTTGGTCAAGAAGA	GCAAAAGCACATGCAGCATA	20	20	50.0	45.0	58.0	58.0	0.0	7.8	0.019695	43978	44652	1005-1005	0.333333	53.2
petA-chlI_p2	petA-chlI	2	GTAATCGTGGTCGTGGTCAA	GCAAAAGCACATGCAGCATA	20	20	50.0	45.0	57.9	58.0	0.0	7.8	0.069789	43978	44652	1247-1247	0.333333	52.8
petA-chlI_p3	petA-chlI	3	GGTGGCTTTGGTCAAGAAGA	TCCGGCTCGATTTGTATTGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.070639	43978	44652	1345-1345	0.333333	52.8
petA-chlI_p4	petA-chlI	4	TTGGTGCTGATGGTAAACCC	GCAAAAGCACATGCAGCATA	20	20	50.0	45.0	58.1	58.0	0.0	7.8	0.083080	43978	44652	1523-1523	0.333333	52.7
petA-chlI_p5	petA-chlI	5	GTTGTTGCACCTGATCCAGA	GCAAAAGCACATGCAGCATA	20	20	50.0	45.0	58.1	58.0	0.0	7.8	0.104732	43978	44652	1314-1314	0.333333	52.5
ftsH-trnT-UGU_p1	ftsH-trnT-UGU	1	CTTGTACGATGGCATTCCGA	AACCCCCAACCTACGGATTA	20	20	50.0	50.0	58.1	58.0	14.5	0.0	0.095699	48403	48659	551-556	0.666667	74.8
ftsH-trnT-UGU_p2	ftsH-trnT-UGU	2	CTTGTACGATGGCATTCCGA	CGACCTTGGGCTTATGAGTC	20	20	50.0	55.0	58.1	58.1	14.5	0.0	0.124086	48403	48659	1065-1163	0.666667	74.1
ftsH-trnT-UGU_p3	ftsH-trnT-UGU	3	CTGGGCTAGAAGCTTGTACG	AACCCCCAACCTACGGATTA	20	20	55.0	50.0	58.1	58.0	2.1	0.0	0.102392	48403	48659	568-568	0.333333	52.5
ftsH-trnT-UGU_p4	ftsH-trnT-UGU	4	CTGGGCTAGAAGCTTGTACG	CGACCTTGGGCTTATGAGTC	20	20	55.0	55.0	58.1	58.1	2.1	0.0	0.130780	48403	48659	1077-1077	0.333333	52.3
ftsH-trnT-UGU_p5	ftsH-trnT-UGU	5	GGAGATTGACCTGAACCACC	AACCCCCAACCTACGGATTA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.145630	48403	48659	797-797	0.333333	52.2
trnS-GCU-trnD-GUC_p1	trnS-GCU-trnD-GUC	1	AATGGCTGAGTGGTCGAAAG	AGACTCGAACTCGCAACTTC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.239769	49362	49432	216-218	1.000000	85.1
trnS-GCU-trnD-GUC_p2	trnS-GCU-trnD-GUC	2	AATGGCTGAGTGGTCGAAAG	GAGACTCGAACTCGCAACTT	20	20	50.0	50.0	58.2	57.9	0.0	4.0	0.239769	49362	49432	217-219	1.000000	85.1
trnS-GCU-trnD-GUC_p3	trnS-GCU-trnD-GUC	3	GAAATGGCTGAGTGGTCGAA	AGACTCGAACTCGCAACTTC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.244871	49362	49432	218-220	1.000000	85.0
trnS-GCU-trnD-GUC_p4	trnS-GCU-trnD-GUC	4	GAAATGGCTGAGTGGTCGAA	GAGACTCGAACTCGCAACTT	20	20	50.0	50.0	58.2	57.9	0.0	4.0	0.244871	49362	49432	219-221	1.000000	85.0
trnS-GCU-trnD-GUC_p5	trnS-GCU-trnD-GUC	5	GGAGAAATGGCTGAGTGGTC	AGACTCGAACTCGCAACTTC	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.309286	49362	49432	221-223	1.000000	84.4
trnD-GUC-petN_p1	trnD-GUC-petN	1	AAGTTGCGAGTTCGAGTCTC	GCCAGCCCAACCTAGACTTA	20	20	50.0	55.0	57.9	59.1	5.6	0.0	1.143659	49507	49700	234-254	1.000000	82.9
trnD-GUC-petN_p2	trnD-GUC-petN	2	GAAGTTGCGAGTTCGAGTCT	GCCAGCCCAACCTAGACTTA	20	20	50.0	55.0	57.9	59.1	5.8	0.0	1.143659	49507	49700	235-255	1.000000	82.9
trnD-GUC-petN_p3	trnD-GUC-petN	3	GAGTTCGAGTCTCGTCAGTC	GCCAGCCCAACCTAGACTTA	20	20	55.0	55.0	57.5	59.1	21.2	0.0	1.604341	49507	49700	227-247	1.000000	79.2
trnD-GUC-petN_p4	trnD-GUC-petN	4	TCAATTGGTTAGAGCACCGC	GCCAGCCCAACCTAGACTTA	20	20	50.0	55.0	58.6	59.1	5.4	0.0	1.641392	49507	49700	267-287	1.000000	78.9
trnD-GUC-petN_p5	trnD-GUC-petN	5	AAGTTGCGAGTTCGAGTCTC	ATTACGCCAGCCCAACCTA	20	19	50.0	52.6	57.9	58.7	5.6	0.0	1.750239	49507	49700	239-259	1.000000	78.0
psaJ-trnP-UGG_p1	psaJ-trnP-UGG	1	TGGTTGGATTGGATGGGTTG	GGTAGAGTACCTGCTTTGGG	20	20	50.0	55.0	58.1	57.3	0.0	0.0	0.757467	64492	64627	556-561	1.000000	82.5
psaJ-trnP-UGG_p2	psaJ-trnP-UGG	2	TTGGATTGGATGGGTTGGAC	GGTAGAGTACCTGCTTTGGG	20	20	50.0	55.0	57.8	57.3	0.0	0.0	0.923396	64492	64627	558-558	0.666667	65.6
psaJ-trnP-UGG_p3	psaJ-trnP-UGG	3	TGGATGACATTTACGGCAGG	GGTAGAGTACCTGCTTTGGG	20	20	50.0	55.0	58.0	57.3	0.0	0.0	0.730224	64492	64627	267-267	0.333333	47.5
psaJ-trnP-UGG_p4	psaJ-trnP-UGG	4	ATGTAGTGAGTCTCCAGCGT	GGTAGAGTACCTGCTTTGGG	20	20	50.0	55.0	58.2	57.3	0.0	0.0	0.852530	64492	64627	822-822	0.333333	46.5
psaJ-trnP-UGG_p5	psaJ-trnP-UGG	5	CTCCACCAGCTCTAGCCTTA	GGTAGAGTACCTGCTTTGGG	20	20	55.0	55.0	58.2	57.3	0.0	0.0	0.911480	64492	64627	736-736	0.333333	46.0
trnP-UGG-ycf89_p1	trnP-UGG-ycf89	1	TGGATGACATTTACGGCAGG	ACCCAATCTACGGCATCAAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.075344	64702	65407	1536-1536	0.333333	52.7
trnP-UGG-ycf89_p2	trnP-UGG-ycf89	2	TGGTTGGATTGGATGGGTTG	ACCCAATCTACGGCATCAAC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.102587	64702	65407	1830-1830	0.333333	52.5
trnP-UGG-ycf89_p3	trnP-UGG-ycf89	3	TGGATGACATTTACGGCAGG	TTGTGCTTGATTTGGGGGAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.131058	64702	65407	1198-1198	0.333333	52.3
trnP-UGG-ycf89_p4	trnP-UGG-ycf89	4	AGGATTTGAACCTACGGCAC	ACCCAATCTACGGCATCAAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.147812	64702	65407	1315-1315	0.333333	52.2
trnP-UGG-ycf89_p5	trnP-UGG-ycf89	5	TGGTTGGATTGGATGGGTTG	TTGTGCTTGATTTGGGGGAA	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.158301	64702	65407	1492-1492	0.333333	52.1
ycf89-rns_p1	ycf89-rns	1	GTTGATGCCGTAGATTGGGT	CATTGCGGAAAATTCCCCAC	20	20	50.0	50.0	58.0	58.0	0.0	6.6	0.047139	66371	66861	1282-1282	0.333333	53.0
ycf89-rns_p2	ycf89-rns	2	GTTGATGCCGTAGATTGGGT	TCTCGTACGACTTGCATGTG	20	20	50.0	50.0	58.0	58.0	0.0	15.3	0.061199	66371	66861	1000-1000	0.333333	52.8
ycf89-rns_p3	ycf89-rns	3	GTTGATGCCGTAGATTGGGT	TATTGCTCCGTCAGGCTTTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.062562	66371	66861	1305-1305	0.333333	52.8
ycf89-rns_p4	ycf89-rns	4	GTTGATGCCGTAGATTGGGT	CAACCCATAGGCAGTCTTCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.082510	66371	66861	1334-1334	0.333333	52.7
ycf89-rns_p5	ycf89-rns	5	GTTGATGCCGTAGATTGGGT	CTTGCATCCTCCGTCTTACC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.097062	66371	66861	1433-1433	0.333333	52.6
ccsA_p1	ccsA	1	GCTGTTTCGCAACGTTTACA	GACCAGTGGATGAAGTTGGA	20	20	45.0	50.0	57.9	57.1	0.0	18.4	0.953137	82159	83136	1618-1618	0.333333	45.7
ccsA_p2	ccsA	2	GCTGTTTCGCAACGTTTACA	AGACCAGTGGATGAAGTTGG	20	20	45.0	50.0	57.9	57.1	0.0	18.4	0.961081	82159	83136	1619-1619	0.333333	45.6
ccsA_p3	ccsA	3	GCAACGTTTACACCCAACAC	GACCAGTGGATGAAGTTGGA	20	20	50.0	50.0	58.2	57.1	0.0	18.4	1.029079	82159	83136	1610-1610	0.333333	45.1
ccsA_p4	ccsA	4	GCAACGTTTACACCCAACAC	AGACCAGTGGATGAAGTTGG	20	20	50.0	50.0	58.2	57.1	0.0	18.4	1.037023	82159	83136	1611-1611	0.333333	45.0
ccsA_p5	ccsA	5	CAACCATCCCAAGGAACCAT	GACCAGTGGATGAAGTTGGA	20	20	50.0	50.0	57.8	57.1	0.0	18.4	1.095654	82159	83136	1549-1549	0.333333	44.6
thiS-psb28_p1	thiS-psb28	1	TCACAACCCACTGCCTTAAC	CTTTGACTCCGCCATTCGTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028923	84755	85042	1061-1139	1.000000	91.8
thiS-psb28_p2	thiS-psb28	2	TCACAACCCACTGCCTTAAC	TTTGACTCCGCCATTCGTAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028923	84755	85042	1060-1138	1.000000	91.8
thiS-psb28_p3	thiS-psb28	3	GTGTTGACCAGGTCTAGCAG	CTTTGACTCCGCCATTCGTA	20	20	55.0	50.0	57.9	58.0	20.8	0.0	0.090637	84755	85042	1266-1320	0.666667	77.9
thiS-psb28_p4	thiS-psb28	4	GTGTTGACCAGGTCTAGCAG	TTTGACTCCGCCATTCGTAG	20	20	55.0	50.0	57.9	58.0	20.8	0.0	0.090637	84755	85042	1265-1319	0.666667	77.9
thiS-psb28_p5	thiS-psb28	5	TCACAACCCACTGCCTTAAC	TGGAACGCTATGCCAAAAGT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.120053	84755	85042	673-673	0.333333	52.4
trnQ-UUG-trnR-ACG_p1	trnQ-UUG-trnR-ACG	1	AAAGTCCACAGCCTTACCAC	GGTTCGAATCCCTTCTTGCT	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.387287	85555	85618	113-122	1.000000	88.5
trnQ-UUG-trnR-ACG_p2	trnQ-UUG-trnR-ACG	2	CAAAGTCCACAGCCTTACCA	GGTTCGAATCCCTTCTTGCT	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.385695	85555	85618	114-123	1.000000	88.4
trnQ-UUG-trnR-ACG_p3	trnQ-UUG-trnR-ACG	3	GAGTCAAAGTCCACAGCCTT	GGTTCGAATCCCTTCTTGCT	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.377766	85555	85618	118-127	1.000000	88.3
trnQ-UUG-trnR-ACG_p4	trnQ-UUG-trnR-ACG	4	GAGTCAAAGTCCACAGCCTT	GGGGTTCGAATCCCTTCTTG	20	20	50.0	55.0	57.7	58.2	0.0	35.1	0.510986	85555	85618	120-129	1.000000	87.1
trnQ-UUG-trnR-ACG_p5	trnQ-UUG-trnR-ACG	5	GTGGCAGGATTCGAACCTAC	GGTTCGAATCCCTTCTTGCT	20	20	55.0	50.0	58.3	57.9	15.9	0.0	0.462191	85555	85618	146-155	1.000000	86.2
rpl6_p1	rpl6	1	CGTCGTAAACGTTGGTCACA	TCATTACGGCGGGATGTTTT	20	20	50.0	45.0	58.8	57.9	0.0	13.2	0.918060	96939	97478	1527-1529	1.000000	79.3
rpl6_p2	rpl6	2	CGTCGTAAACGTTGGTCACA	TTCATTACGGCGGGATGTTT	20	20	50.0	45.0	58.8	57.9	0.0	13.2	0.918060	96939	97478	1528-1530	1.000000	79.2
rpl6_p3	rpl6	3	CGTCGTAAACGTTGGTCACA	TTTCATTACGGCGGGATGTT	20	20	50.0	45.0	58.8	57.9	0.0	13.2	0.918060	96939	97478	1529-1531	1.000000	79.2
rpl6_p4	rpl6	4	CGTCGTAAACGTTGGTCACA	CCCCATAAGTCTGGAAGCAC	20	20	50.0	55.0	58.8	58.0	0.0	0.0	0.845356	96939	97478	1316-1318	0.666667	65.3
rpl6_p5	rpl6	5	CGTCGTAAACGTTGGTCACA	AAATTGTAGGCCACCTGCTC	20	20	50.0	50.0	58.8	58.2	0.0	5.0	0.961810	96939	97478	1444-1444	0.333333	45.6
ccsA_p1	ccsA	1	CTCCCCAGGTAGGACTTGAA	GCTGTTTCGCAACGTTTACA	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.141350	110525	111502	1966-1968	1.000000	87.0
ccsA_p2	ccsA	2	CTCCCCAGGTAGGACTTGAA	GCAACGTTTACACCCAACAC	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.217292	110525	111502	1958-1960	1.000000	86.3
ccsA_p3	ccsA	3	AGGACTTGAACCTACGACCA	GCTGTTTCGCAACGTTTACA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.099342	110525	111502	1958-1958	0.333333	52.5
ccsA_p4	ccsA	4	AGGACTTGAACCTACGACCA	GCAACGTTTACACCCAACAC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.175284	110525	111502	1950-1950	0.333333	51.9
ccsA_p5	ccsA	5	AGGACTTGAACCTACGACCA	CAACCATCCCAAGGAACCAT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.241859	110525	111502	1889-1889	0.333333	51.4
rns-ycf89_p1	rns-ycf89	1	CATTGCGGAAAATTCCCCAC	GTTGATGCCGTAGATTGGGT	20	20	50.0	50.0	58.0	58.0	6.6	0.0	0.047139	126800	127290	1282-1282	0.333333	53.0
rns-ycf89_p2	rns-ycf89	2	TCTCGTACGACTTGCATGTG	GTTGATGCCGTAGATTGGGT	20	20	50.0	50.0	58.0	58.0	15.3	0.0	0.061199	126800	127290	1000-1000	0.333333	52.8
rns-ycf89_p3	rns-ycf89	3	TATTGCTCCGTCAGGCTTTC	GTTGATGCCGTAGATTGGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.062562	126800	127290	1305-1305	0.333333	52.8
rns-ycf89_p4	rns-ycf89	4	CAACCCATAGGCAGTCTTCC	GTTGATGCCGTAGATTGGGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.082510	126800	127290	1334-1334	0.333333	52.7
rns-ycf89_p5	rns-ycf89	5	CTTGCATCCTCCGTCTTACC	GTTGATGCCGTAGATTGGGT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.097062	126800	127290	1433-1433	0.333333	52.6
ycf89-trnP-UGG_p1	ycf89-trnP-UGG	1	TGTGCTTGATTTGGGGGAAT	AGGATTTGAACCTACGGCAC	20	20	45.0	50.0	57.7	57.9	0.0	0.0	0.433423	128254	128959	816-976	0.666667	75.2
ycf89-trnP-UGG_p2	ycf89-trnP-UGG	2	ACCCAATCTACGGCATCAAC	AGGATTTGAACCTACGGCAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.147812	128254	128959	1315-1315	0.333333	52.2
ycf89-trnP-UGG_p3	ycf89-trnP-UGG	3	TTGTGCTTGATTTGGGGGAA	AGGATTTGAACCTACGGCAC	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.203525	128254	128959	977-977	0.333333	51.7
ycf89-trnP-UGG_p4	ycf89-trnP-UGG	4	TGGCATTCCAGGAACATCTG	AGGATTTGAACCTACGGCAC	20	20	50.0	50.0	57.9	57.9	3.2	0.0	0.242706	128254	128959	884-884	0.333333	51.4
ycf89-trnP-UGG_p5	ycf89-trnP-UGG	5	ATTGTGCTTGATTTGGGGGA	AGGATTTGAACCTACGGCAC	20	20	45.0	50.0	57.7	57.9	0.0	0.0	0.433423	128254	128959	978-978	0.333333	49.9