Hotspot

rpl16-rps3

LSC · 90359-91826 · 1468 bp

Back to Discocleidion

Pi 0.0049

MarkerSeek score 64.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1468
variable_sites: 7
pi: 0.0049
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GTCGTCCCGCTTCTATTTGT	TCCTTTACAAACCATTCGAGCT	50.0	40.9	58.0	58.0	1676-1683 bp	1.0	66.0
rpl16-rps3_p2	GTCGTCCCGCTTCTATTTGT	GGTAGGGTTCCTTTACAAACCA	50.0	45.5	58.0	57.9	1684-1691 bp	1.0	65.3
rpl16-rps3_p3	GTCGTCCCGCTTCTATTTGT	GAGGGTAGGGTTCCTTTACA	50.0	50.0	58.0	55.8	1687-1694 bp	1.0	64.8
rpl16-rps3_p4	GTCGTCCCGCTTCTATTTGT	GGGTTCCTTTACAAACCATTCG	50.0	45.5	58.0	57.8	1680-1687 bp	1.0	64.7
rpl16-rps3_p5	TCGTCCCGCTTCTATTTGTC	TCCTTTACAAACCATTCGAGCT	50.0	40.9	57.7	58.0	1675-1682 bp	1.0	63.7