Hotspot

accD-psaI

LSC · 64807-66381 · 1575 bp

Back to Discocleidion

Pi 0.0285

MarkerSeek score 74.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1575
variable_sites: 36
pi: 0.0285
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCGTTAAAAGGCGTTCTGA	AAAGAAGCCATCGCAATTGC	45.0	45.0	57.5	58.0	1474-1734 bp	1.0	81.6
accD-psaI_p2	TCCGTTAAAAGGCGTTCTGA	TTGCCGGAAATACTAAGCCC	45.0	50.0	57.5	58.0	1458-1718 bp	1.0	81.2
accD-psaI_p3	TCCGTTAAAAGGCGTTCTGA	AAGGCACAAAAATGGAGGGT	45.0	45.0	57.5	57.9	1434-1694 bp	1.0	80.8
accD-psaI_p4	TGAGTTACTTCAGCTCCACG	AAAGAAGCCATCGCAATTGC	50.0	45.0	57.3	58.0	1453-1713 bp	1.0	80.0
accD-psaI_p5	TGAGTTACTTCAGCTCCACG	TTGCCGGAAATACTAAGCCC	50.0	50.0	57.3	58.0	1437-1697 bp	1.0	79.7