Hotspot

psaA-ycf3

LSC · 47843-48634 · 792 bp

Back to Discocleidion

Pi 0.00656

MarkerSeek score 66.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 5
pi: 0.00656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCAGAGTATTGG	50.0	50.0	57.9	58.0	940-970 bp	1.0	81.7
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	882-912 bp	1.0	81.4
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCAGAGTATT	50.0	50.0	57.9	58.1	942-972 bp	1.0	80.9
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGCCGCAGAGTATTGGAAAC	50.0	50.0	57.9	58.3	936-966 bp	1.0	79.9
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	CAAGCCGCAGAGTATTGGAA	50.0	50.0	57.9	58.3	938-968 bp	1.0	79.9