Hotspot

trnG-UCC-trnfM-CAU

LSC · 42497-42696 · 200 bp

Back to Discocleidion

Pi 0.0156

MarkerSeek score 65.30

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 200
variable_sites: 3
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	AACCTTGAAGTCACGGGTTC	50.0	50.0	57.8	58.0	273-281 bp	1.0	81.5
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	CCTTGAAGTCACGGGTTCAA	50.0	50.0	57.8	58.0	271-279 bp	1.0	81.5
trnG-UCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	AAGTCACGGGTTCAAATCCC	50.0	50.0	57.8	58.1	266-274 bp	1.0	81.1
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	CTCTTCATTACGCGGGGTAG	50.0	55.0	57.8	57.9	320-328 bp	1.0	80.3
trnG-UCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	CTTCATTACGCGGGGTAGAG	50.0	55.0	57.8	57.9	318-326 bp	1.0	80.3