Hotspot

psbC-trnS-UGA

LSC · 40929-41166 · 238 bp

Back to Discocleidion

Pi 0.0294

MarkerSeek score 69.40

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 238
variable_sites: 7
pi: 0.0294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	385 bp	1.0	78.0
psbC-trnS-UGA_p2	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.4	58.1	419 bp	1.0	77.5
psbC-trnS-UGA_p3	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	384 bp	1.0	77.4
psbC-trnS-UGA_p4	AGCTGCAGCAGGATTTGAAA	GATGGCCGAGTGGTTGATAG	45.0	55.0	58.4	58.1	396 bp	1.0	77.4
psbC-trnS-UGA_p5	TAGGTCATTTATGGCACGCG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.4	58.1	430 bp	1.0	76.9