Hotspot

rps16-trnQ-UUG

LSC · 6618-7621 · 1004 bp

Back to Discocleidion

Pi 0.00806
MarkerSeek score 64.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1004
variable_sites
8
pi
0.00806
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ACGTTGCTTTCTACCCCATC GAAATTCAAATGGGGCGTGG 50.0 50.0 57.9 58.0 1123-1128 bp 1.0 80.4
rps16-trnQ-UUG_p2 GTTGCTTTCTACCCCATCGT GAAATTCAAATGGGGCGTGG 50.0 50.0 57.9 58.0 1121-1126 bp 1.0 80.4
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAAATTCAAATGGGGCGTGG 45.0 50.0 58.1 58.0 1130-1135 bp 1.0 80.2
rps16-trnQ-UUG_p4 ACGTTGCTTTCTACCCCATC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1065-1070 bp 1.0 79.9
rps16-trnQ-UUG_p5 GTTGCTTTCTACCCCATCGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1063-1068 bp 1.0 79.9