primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	131	682	581-619	1.000000	83.0
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	131	682	595-633	1.000000	82.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	131	682	594-632	1.000000	82.9
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	131	682	596-634	1.000000	82.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	131	682	584-622	1.000000	82.9
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	AAACGACCCCATAGGCTTTC	CTATCCGACTAGTTCCGGGT	20	20	50.0	55.0	57.9	58.0	0.0	19.6	0.145823	1745	2178	516-523	1.000000	82.1
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	GAAACGACCCCATAGGCTTT	CTATCCGACTAGTTCCGGGT	20	20	50.0	55.0	57.9	58.0	0.0	19.6	0.145823	1745	2178	517-524	1.000000	82.1
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	CCAGTTACAGAAACGACCCC	CTATCCGACTAGTTCCGGGT	20	20	55.0	55.0	58.2	58.0	0.0	19.6	0.216945	1745	2178	526-533	1.000000	81.4
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	AAACGACCCCATAGGCTTTC	CGGCTTGGAGAGGGATTTTT	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.284854	1745	2178	568-576	1.000000	80.8
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	GAAACGACCCCATAGGCTTT	CGGCTTGGAGAGGGATTTTT	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.284854	1745	2178	569-577	1.000000	80.8
trnK-UUU_p1	trnK-UUU	1	AGCATTTCATCTGATTTCAATATGA	TGATTGTGTGATGCGCTTCA	25	20	28.0	45.0	55.4	58.5	0.0	20.9	8.059219	2179	4818	2769-2777	1.000000	40.7
trnK-UUU_p2	trnK-UUU	2	AGCATTTCATCTGATTTCAATATGA	GATTGTGTGATGCGCTTCAA	25	20	28.0	45.0	55.4	57.4	0.0	25.5	8.198049	2179	4818	2768-2776	1.000000	40.7
trnK-UUU_p3	trnK-UUU	3	AGCATTTCATCTGATTTCAATATGA	TTGATTGTGTGATGCGCTTC	25	20	28.0	45.0	55.4	57.4	0.0	15.9	8.198049	2179	4818	2770-2778	1.000000	40.7
trnK-UUU_p4	trnK-UUU	4	GCATTTCATCTGATTTCAATATGA	TGATTGTGTGATGCGCTTCA	24	20	29.2	45.0	54.1	58.5	0.0	20.9	8.409331	2179	4818	2768-2776	1.000000	40.7
trnK-UUU_p5	trnK-UUU	5	AGCATTTCATCTGATTTCAATATG	TGATTGTGTGATGCGCTTCA	24	20	29.2	45.0	54.1	58.5	0.0	20.9	8.422595	2179	4818	2769-2777	1.000000	40.7
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	ACGTTGCTTTCTACCCCATC	GAAATTCAAATGGGGCGTGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.118176	6618	7621	1123-1128	1.000000	80.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GTTGCTTTCTACCCCATCGT	GAAATTCAAATGGGGCGTGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.118176	6618	7621	1121-1126	1.000000	80.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	GAAATTCAAATGGGGCGTGG	20	20	45.0	50.0	58.1	58.0	8.1	0.0	0.148076	6618	7621	1130-1135	1.000000	80.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	ACGTTGCTTTCTACCCCATC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.185526	6618	7621	1065-1070	1.000000	79.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GTTGCTTTCTACCCCATCGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.185526	6618	7621	1063-1068	1.000000	79.9
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CAATCCGACGCTTTAGTCCA	TTCATCGGAATATGGAGCCG	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.460256	9079	10011	625-1056	1.000000	81.7
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AATCCGACGCTTTAGTCCAC	TTCATCGGAATATGGAGCCG	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.461644	9079	10011	624-1055	1.000000	81.6
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	CAATCCGACGCTTTAGTCCA	GAGCCGAGGATCCCTTAACT	20	20	50.0	55.0	58.0	58.6	0.0	8.6	0.589383	9079	10011	611-1042	1.000000	80.6
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	AATCCGACGCTTTAGTCCAC	GAGCCGAGGATCCCTTAACT	20	20	50.0	55.0	58.0	58.6	0.0	8.6	0.590771	9079	10011	610-1041	1.000000	80.6
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	TTCCAAGTCATCACCGGAAC	TTCATCGGAATATGGAGCCG	20	20	50.0	50.0	57.8	57.5	9.5	0.0	0.634586	9079	10011	698-1129	1.000000	80.1
trnG-GCC-trnR-UCU_p1	trnG-GCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	10788	11633	868-926	1.000000	68.1
trnG-GCC-trnR-UCU_p2	trnG-GCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	10788	11633	871-929	1.000000	67.8
trnG-GCC-trnR-UCU_p3	trnG-GCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	10788	11633	874-932	1.000000	67.4
trnG-GCC-trnR-UCU_p4	trnG-GCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	10788	11633	877-935	1.000000	67.2
trnG-GCC-trnR-UCU_p5	trnG-GCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	10788	11633	872-930	1.000000	65.6
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTCCGTAATACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.107610	34052	36689	2779-2779	0.500000	59.1
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTCCGTAATACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.9	58.1	0.0	0.0	0.165707	34052	36689	2758-2758	0.500000	58.7
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTCCGTAATACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.187862	34052	36689	2755-2755	0.500000	58.5
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TTCCGTAATACCCTACCCCC	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	57.9	58.2	0.0	0.0	0.252543	34052	36689	2776-2776	0.500000	58.0
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	34052	36689	2741-2741	0.500000	57.8
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	58.4	58.1	38.2	0.0	0.434339	40929	41166	385-385	1.000000	78.0
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	58.4	58.1	3.5	0.0	0.481207	40929	41166	419-419	1.000000	77.5
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	57.6	58.1	33.4	0.0	0.502562	40929	41166	384-384	1.000000	77.4
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	AGCTGCAGCAGGATTTGAAA	GATGGCCGAGTGGTTGATAG	20	20	45.0	55.0	58.4	58.1	38.2	0.0	0.504584	40929	41166	396-396	1.000000	77.4
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	TAGGTCATTTATGGCACGCG	GATGGCCGAGTGGTTGATAG	20	20	50.0	55.0	58.4	58.1	3.5	0.0	0.551451	40929	41166	430-430	1.000000	76.9
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	CCGGTACATCATTATGGATTGGA	CGTCTTCTCCTTGGCAAAGA	23	20	43.5	50.0	58.1	57.8	0.0	1.1	3.264404	41808	42425	711-730	1.000000	59.0
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	TCCGGTACATCATTATGGATTGG	CGTCTTCTCCTTGGCAAAGA	23	20	43.5	50.0	58.1	57.8	0.0	1.1	3.264404	41808	42425	712-731	1.000000	59.0
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	CCGGTACATCATTATGGATTGG	CGTCTTCTCCTTGGCAAAGA	22	20	45.5	50.0	56.7	57.8	0.0	1.1	3.467182	41808	42425	711-730	1.000000	57.4
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	CCGGTACATCATTATGGATTGGA	CGGATAGCGGGAATCGAAC	23	19	43.5	57.9	58.1	58.2	0.0	0.0	4.284085	41808	42425	733-752	1.000000	50.8
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	TCCGGTACATCATTATGGATTGG	CGGATAGCGGGAATCGAAC	23	19	43.5	57.9	58.1	58.2	0.0	0.0	4.284085	41808	42425	734-753	1.000000	50.8
trnG-UCC-trnfM-CAU_p1	trnG-UCC-trnfM-CAU	1	TCTTTGCCAAGGAGAAGACG	AACCTTGAAGTCACGGGTTC	20	20	50.0	50.0	57.8	58.0	0.0	8.5	0.206660	42497	42696	273-281	1.000000	81.5
trnG-UCC-trnfM-CAU_p2	trnG-UCC-trnfM-CAU	2	TCTTTGCCAAGGAGAAGACG	CCTTGAAGTCACGGGTTCAA	20	20	50.0	50.0	57.8	58.0	0.0	3.7	0.208039	42497	42696	271-279	1.000000	81.5
trnG-UCC-trnfM-CAU_p3	trnG-UCC-trnfM-CAU	3	TCTTTGCCAAGGAGAAGACG	AAGTCACGGGTTCAAATCCC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.264195	42497	42696	266-274	1.000000	81.1
trnG-UCC-trnfM-CAU_p4	trnG-UCC-trnfM-CAU	4	TCTTTGCCAAGGAGAAGACG	CTCTTCATTACGCGGGGTAG	20	20	50.0	55.0	57.8	57.9	0.0	12.5	0.305497	42497	42696	320-328	1.000000	80.3
trnG-UCC-trnfM-CAU_p5	trnG-UCC-trnfM-CAU	5	TCTTTGCCAAGGAGAAGACG	CTTCATTACGCGGGGTAGAG	20	20	50.0	55.0	57.8	57.9	0.0	12.5	0.305497	42497	42696	318-326	1.000000	80.3
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCAGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	47843	48634	940-970	1.000000	81.7
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	20	20	50.0	45.0	57.9	58.1	4.1	11.5	0.219123	47843	48634	882-912	1.000000	81.4
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCAGAGTATT	20	20	50.0	50.0	57.9	58.1	4.1	0.0	0.257251	47843	48634	942-972	1.000000	80.9
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	AGCCGCAGAGTATTGGAAAC	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.376276	47843	48634	936-966	1.000000	79.9
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	CAAGCCGCAGAGTATTGGAA	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.377504	47843	48634	938-968	1.000000	79.9
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GCCCTTTGCTAATTGGGGAT	GAACGAGCCCAATGCAAAAA	20	20	50.0	45.0	58.2	57.9	7.7	0.0	0.354022	56416	57220	1037-1246	1.000000	81.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GCCCTTTGCTAATTGGGGAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.2	57.8	7.7	0.0	0.403869	56416	57220	950-1159	1.000000	81.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GCCCTTTGCTAATTGGGGAT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.2	57.5	7.7	0.0	0.747641	56416	57220	949-1158	1.000000	78.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GCCCTTTGCTAATTGGGGAT	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	58.2	58.7	7.7	0.0	0.889935	56416	57220	938-1147	1.000000	77.2
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GCCCTTTGCTAATTGGGGAT	AAGGTCTACGGTTCGAGTCC	20	20	50.0	55.0	58.2	58.8	7.7	0.0	1.034411	56416	57220	948-1157	1.000000	76.0
trnV-UAC_p1	trnV-UAC	1	ACATGATGGACTCCCTTGAA	AGCAATACCGATCAAAGAGA	20	20	45.0	40.0	56.1	54.2	0.0	0.0	5.708190	57845	57883	185-186	1.000000	40.4
trnV-UAC_p2	trnV-UAC	2	ACATGATGGACTCCCTTGAA	AAGCAATACCGATCAAAGAGA	20	21	45.0	38.1	56.1	55.0	0.0	0.0	5.848182	57845	57883	186-187	1.000000	40.4
trnV-UAC_p3	trnV-UAC	3	ACATGATGGACTCCCTTGAAA	AGCAATACCGATCAAAGAGA	21	20	42.9	40.0	56.9	54.2	0.0	0.0	5.926806	57845	57883	185-186	1.000000	40.4
trnV-UAC_p4	trnV-UAC	4	AGGCTATTGTTCTCTTGTTCCC	AGCAATACCGATCAAAGAGA	22	20	45.5	40.0	58.0	54.2	0.0	0.0	5.873459	57845	57883	254-255	1.000000	40.3
trnV-UAC_p5	trnV-UAC	5	GGCTATTGTTCTCTTGTTCCCT	AGCAATACCGATCAAAGAGA	22	20	45.5	40.0	58.0	54.2	0.0	0.0	5.873459	57845	57883	253-254	1.000000	40.3
accD-psaI_p1	accD-psaI	1	TCCGTTAAAAGGCGTTCTGA	AAAGAAGCCATCGCAATTGC	20	20	45.0	45.0	57.5	58.0	0.0	20.8	0.530210	64807	66381	1474-1734	1.000000	81.6
accD-psaI_p2	accD-psaI	2	TCCGTTAAAAGGCGTTCTGA	TTGCCGGAAATACTAAGCCC	20	20	45.0	50.0	57.5	58.0	0.0	5.0	0.576782	64807	66381	1458-1718	1.000000	81.2
accD-psaI_p3	accD-psaI	3	TCCGTTAAAAGGCGTTCTGA	AAGGCACAAAAATGGAGGGT	20	20	45.0	45.0	57.5	57.9	0.0	0.0	0.631420	64807	66381	1434-1694	1.000000	80.8
accD-psaI_p4	accD-psaI	4	TGAGTTACTTCAGCTCCACG	AAAGAAGCCATCGCAATTGC	20	20	50.0	45.0	57.3	58.0	0.0	20.8	0.725406	64807	66381	1453-1713	1.000000	80.0
accD-psaI_p5	accD-psaI	5	TGAGTTACTTCAGCTCCACG	TTGCCGGAAATACTAAGCCC	20	20	50.0	50.0	57.3	58.0	0.0	5.0	0.771979	64807	66381	1437-1697	1.000000	79.7
rpl16-rps3_p1	rpl16-rps3	1	GTCGTCCCGCTTCTATTTGT	TCCTTTACAAACCATTCGAGCT	20	22	50.0	40.9	58.0	58.0	0.0	0.0	2.021843	90359	91826	1676-1683	1.000000	66.0
rpl16-rps3_p2	rpl16-rps3	2	GTCGTCCCGCTTCTATTTGT	GGTAGGGTTCCTTTACAAACCA	20	22	50.0	45.5	58.0	57.9	0.0	6.6	2.108984	90359	91826	1684-1691	1.000000	65.3
rpl16-rps3_p3	rpl16-rps3	3	GTCGTCCCGCTTCTATTTGT	GAGGGTAGGGTTCCTTTACA	20	20	50.0	50.0	58.0	55.8	0.0	0.0	2.163634	90359	91826	1687-1694	1.000000	64.8
rpl16-rps3_p4	rpl16-rps3	4	GTCGTCCCGCTTCTATTTGT	GGGTTCCTTTACAAACCATTCG	20	22	50.0	45.5	58.0	57.8	0.0	6.6	2.174893	90359	91826	1680-1687	1.000000	64.7
rpl16-rps3_p5	rpl16-rps3	5	TCGTCCCGCTTCTATTTGTC	TCCTTTACAAACCATTCGAGCT	20	22	50.0	40.9	57.7	58.0	0.0	0.0	2.304833	90359	91826	1675-1682	1.000000	63.7
ycf1-ndhF_p1	ycf1-ndhF	1	ACGGAAGAAAGGGAGGAAGA	GCGGTCGCATCTCTTCTTAT	20	20	50.0	50.0	58.0	57.9	0.0	10.7	0.167171	120131	121307	1527-1871	1.000000	87.1
ycf1-ndhF_p2	ycf1-ndhF	2	CGGAAGAAAAGGAGGATCGG	GCGGTCGCATCTCTTCTTAT	20	20	55.0	50.0	58.1	57.9	0.0	10.7	0.200210	120131	121307	1421-1765	1.000000	87.1
ycf1-ndhF_p3	ycf1-ndhF	3	ACGGAAGAAAGGGAGGAAGA	AATATGTAGGAAGCGGTCGC	20	20	50.0	50.0	58.0	57.8	0.0	7.3	0.176033	120131	121307	1539-1883	1.000000	87.0
ycf1-ndhF_p4	ycf1-ndhF	4	GCAGAATACCGTCACCCATT	GCGGTCGCATCTCTTCTTAT	20	20	50.0	50.0	58.0	57.9	0.0	10.7	0.179922	120131	121307	1577-1921	1.000000	86.9
ycf1-ndhF_p5	ycf1-ndhF	5	GCAGAATACCGTCACCCATT	AATATGTAGGAAGCGGTCGC	20	20	50.0	50.0	58.0	57.8	0.0	7.3	0.188784	120131	121307	1589-1933	1.000000	86.8