Hotspot

ycf4

LSC · 66926-67492 · 567 bp

Back to Dischidia

Pi 0.0359

MarkerSeek score 70.30

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 567
variable_sites: 57
pi: 0.0359
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	ATTTCCGGCAATTGCAATGG	GAGAGAGAAAGGCACCAAGG	45.0	55.0	58.0	57.9	1751-1787 bp	1.0	86.3
ycf4_p2	AATCGACCAGGATCGCTAGA	TTCACTGATTGCCCACAGAC	50.0	50.0	58.0	58.1	1090-1127 bp	0.666667	70.1
ycf4_p3	GCCGTTTTCAAATCGACCAG	TTCACTGATTGCCCACAGAC	50.0	50.0	58.0	58.1	1100-1137 bp	0.666667	70.0
ycf4_p4	AATCGACCAGGATCGCTAGA	GAGAGAGAAAGGCACCAAGG	50.0	55.0	58.0	57.9	1498-1537 bp	0.666667	69.7
ycf4_p5	ATTTCCGGCAATTGCAATGG	TTCACTGATTGCCCACAGAC	45.0	50.0	58.0	58.1	1343-1377 bp	0.666667	69.3