Hotspot

accD

LSC · 64044-66083 · 2040 bp

Back to Dischidia

Pi 0.0313

MarkerSeek score 61.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 2040
variable_sites: 127
pi: 0.0313
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATCCTTGGGATGGGGCTATT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	2642-2996 bp	1.0	87.4
accD_p2	ACGACATAAGAAAAGGCGCT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	2710-2797 bp	0.666667	72.0
accD_p3	ACGACATAAGAAAAGGCGCT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.9	57.9	2716-2803 bp	0.666667	71.4
accD_p4	ACGACATAAGAAAAGGCGCT	GGGGGTCCATCAGGTCTAAA	45.0	55.0	57.9	58.4	2766-2853 bp	0.666667	68.9
accD_p5	ACGACATAAGAAAAGGCGCT	GCCGGAAATACTAGGCCTAC	45.0	55.0	57.9	57.5	2699-2786 bp	0.666667	68.6