Hotspot

ycf1-trnN-GUU

IRa · 152363-152777 · 415 bp

Back to Dischidia

Pi 0.0169

MarkerSeek score 58.70

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 415
variable_sites: 14
pi: 0.0169
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	ATAGAGTCCGACCACAACGA	AGGATTCGAACCTACGACCA	50.0	50.0	58.2	58.2	561-776 bp	1.0	83.1
ycf1-trnN-GUU_p2	ACCACAACGACCGAATTGAT	AGGATTCGAACCTACGACCA	45.0	50.0	57.8	58.2	551-766 bp	1.0	83.1
ycf1-trnN-GUU_p3	ATAGAGTCCGACCACAACGA	CCCCAAGTAGGATTCGAACC	50.0	55.0	58.2	57.7	569-784 bp	1.0	81.8
ycf1-trnN-GUU_p4	ACCACAACGACCGAATTGAT	CCCCAAGTAGGATTCGAACC	45.0	55.0	57.8	57.7	559-774 bp	1.0	81.7
ycf1-trnN-GUU_p5	GACCACAACGACCGAATTGA	AGGATTCGAACCTACGACCA	50.0	50.0	58.5	58.2	552-767 bp	1.0	80.6