Hotspot

ndhF-rpl32

IRa · 135052-135627 · 576 bp

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Pi 0.0262

MarkerSeek score 59.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: IRa
length_bp: 576
variable_sites: 34
pi: 0.0262
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAAGCGTTTTGGCAAGCAT	GGAAAAACCCTCTCCCCATC	40.0	55.0	58.0	57.9	1604-1635 bp	1.0	82.9
ndhF-rpl32_p2	AAAGCGTTTTGGCAAGCATT	GGAAAAACCCTCTCCCCATC	40.0	55.0	58.0	57.9	1603-1634 bp	1.0	82.9
ndhF-rpl32_p3	AAGCGTTTTGGCAAGCATTT	GGAAAAACCCTCTCCCCATC	40.0	55.0	58.0	57.9	1602-1633 bp	1.0	82.9
ndhF-rpl32_p4	TGGAAAGACCATATGCGACG	GGAAAAACCCTCTCCCCATC	50.0	55.0	58.1	57.9	1195-1226 bp	1.0	82.6
ndhF-rpl32_p5	TCGGAAAAAGAAGAAGCCCC	GGAAAAACCCTCTCCCCATC	50.0	55.0	58.1	57.9	1158-1189 bp	1.0	82.2