Hotspot

rps19-rpl2

IRb · 91661-91727 · 67 bp

Back to Dischidia

Pi 0.0259

MarkerSeek score 57.70

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 67
variable_sites: 3
pi: 0.0259
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGCTCGGGACCAAGTTACTA	TTCTTCGTCGCCGTAGTAAA	50.0	45.0	58.1	57.0	199-208 bp	1.0	74.3
rps19-rpl2_p2	TGCTCGGGACCAAGTTACTA	ATTCTTCGTCGCCGTAGTAA	50.0	45.0	58.1	56.8	200-209 bp	1.0	72.5
rps19-rpl2_p3	TGCTCGGGACCAAGTTACTA	TCTTCGTCGCCGTAGTAAAT	50.0	45.0	58.1	56.8	198-207 bp	1.0	72.5
rps19-rpl2_p4	TGCTCGGGACCAAGTTACTA	TTATTCTTCGTCGCCGTAGT	50.0	45.0	58.1	56.8	202-211 bp	1.0	72.5
rps19-rpl2_p5	GATGCTCGGGACCAAGTTAC	TTCTTCGTCGCCGTAGTAAA	55.0	45.0	58.3	57.0	201-210 bp	1.0	72.1