Hotspot

rbcL-accD

LSC · 63220-64043 · 824 bp

Back to Dischidia

Pi 0.019

MarkerSeek score 61.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 36
pi: 0.019
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	ACCACCAGTTTTTCATAGAGT	50.0	38.1	57.9	54.7	979-1008 bp	1.0	52.0
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	ACCACCAGTTTTTCATAGAGTT	50.0	36.4	57.9	55.6	979-1008 bp	1.0	50.4
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	AGAGTTATTTTGTCCCCCTT	50.0	40.0	57.9	53.8	963-992 bp	0.666667	38.3
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	TCATAGAGTTATTTTGTCCCCCT	50.0	39.1	57.9	56.7	967-996 bp	0.666667	37.5
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	AGAGTTATTTTGTCCCCCTTT	50.0	38.1	57.9	54.7	963-992 bp	0.666667	37.5