Hotspot

trnT-UGU-trnL-UAA

LSC · 52472-53281 · 810 bp

Back to Dischidia

Pi 0.017

MarkerSeek score 64.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 32
pi: 0.017
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	TCTGCCGATTTCGCCATATC	55.0	50.0	57.9	58.2	853-892 bp	1.0	82.0
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	ATTAAGTCCGTAGCGTCTGC	55.0	50.0	57.9	57.8	868-907 bp	1.0	81.9
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	TCTGCCGATTTCGCCATATC	50.0	50.0	57.8	58.2	885-924 bp	1.0	81.0
trnT-UGU-trnL-UAA_p4	TAGGAAAAAGCCGGCTATCG	TCTGCCGATTTCGCCATATC	50.0	50.0	57.8	58.2	904-943 bp	1.0	80.9
trnT-UGU-trnL-UAA_p5	TAGGAAAAAGCCGGCTATCG	ATTAAGTCCGTAGCGTCTGC	50.0	50.0	57.8	57.8	919-958 bp	1.0	80.9