Hotspot

rps2-rpoC2

LSC · 16794-21719 · 4926 bp

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Pi 0.0135

MarkerSeek score 49.30

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 4926
variable_sites: 153
pi: 0.0135
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CTTAGCATACCAAGGGGCTG	CGGCATTCTGTTCTACAGCT	55.0	50.0	58.0	58.0	689-838 bp	1.0	89.3
rps2-rpoC2_p2	CTTAGCATACCAAGGGGCTG	CTCCGGGGGCTATTAGAGAA	55.0	55.0	58.0	58.0	1156-1305 bp	1.0	87.9
rps2-rpoC2_p3	CTTAGCATACCAAGGGGCTG	TCCGGGGGCTATTAGAGAAG	55.0	55.0	58.0	58.0	1155-1304 bp	1.0	87.9
rps2-rpoC2_p4	CTTAGCATACCAAGGGGCTG	AGAGAAGGAGCTTCGTTCCT	55.0	50.0	58.0	58.1	1142-1291 bp	1.0	87.4
rps2-rpoC2_p5	CTTAGCATACCAAGGGGCTG	CGGGGGCTATTAGAGAAGGA	55.0	55.0	58.0	58.0	1168-1213 bp	0.5	63.3