Hotspot

ycf1

SSC · 127203-132863 · 5661 bp

Back to Disanthus

Pi 0.000353

MarkerSeek score 52.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5661
variable_sites: 2
pi: 0.000353
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGTTGTGCATTCATCTTGGG	ACCGACAGAACAAGCACAAT	50.0	45.0	57.7	58.0	287 bp	1.0	77.9
ycf1_p2	CGTTGTGCATTCATCTTGGG	GACCGACAGAACAAGCACAA	50.0	50.0	57.7	58.7	288 bp	1.0	72.5
ycf1_p3	ACGTTGTGCATTCATCTTGG	ACCGACAGAACAAGCACAAT	45.0	45.0	57.0	58.0	288 bp	1.0	72.1
ycf1_p4	GGTTCAAGAAAACGTTGTGCA	ACCGACAGAACAAGCACAAT	42.9	45.0	58.1	58.0	299 bp	1.0	71.0
ycf1_p5	TGGTTCAAGAAAACGTTGTGC	ACCGACAGAACAAGCACAAT	42.9	45.0	58.1	58.0	300 bp	1.0	71.0