Hotspot

rpoC2

LSC · 17599-21756 · 4158 bp

Back to Disanthus

Pi 0.000241

MarkerSeek score 54.00

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4158
variable_sites: 1
pi: 0.000241
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GCACCAATCAAGAATCCCCA	TGTTCATGGCCATTATGGGG	50.0	50.0	58.1	57.9	228 bp	1.0	78.5
rpoC2_p2	AGCACCAATCAAGAATCCCC	TGTTCATGGCCATTATGGGG	50.0	50.0	57.9	57.9	229 bp	1.0	78.5
rpoC2_p3	GCACCAATCAAGAATCCCCA	ACACCTAAAAGCGGGTCAAG	50.0	50.0	58.1	58.1	318 bp	1.0	78.2
rpoC2_p4	GCACCAATCAAGAATCCCCA	CACCTAAAAGCGGGTCAAGT	50.0	50.0	58.1	58.1	317 bp	1.0	78.2
rpoC2_p5	AGCACCAATCAAGAATCCCC	CACCTAAAAGCGGGTCAAGT	50.0	50.0	57.9	58.1	318 bp	1.0	78.2