Hotspot

petA

LSC · 64605-65567 · 963 bp

Back to Disanthus

Pi 0.00104

MarkerSeek score 59.30

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	AGCAGAATCGTGGATAGGGA	AAGGCAAAGGCTTTTCATAA	50.0	35.0	57.9	53.6	1136 bp	1.0	44.3
petA_p2	AGCAGAATCGTGGATAGGGA	AGGCAAAGGCTTTTCATAAT	50.0	35.0	57.9	53.4	1135 bp	1.0	42.3
petA_p3	AGCAGAATCGTGGATAGGGA	AAGGCAAAGGCTTTTCATAAT	50.0	33.3	57.9	54.3	1136 bp	1.0	41.6
petA_p4	AGCAGAATCGTGGATAGGGA	AGGCAAAGGCTTTTCATAATT	50.0	33.3	57.9	54.3	1135 bp	1.0	41.6
petA_p5	AGCAGAATCGTGGATAGGGA	AGGCAAAGGCTTTTCATAATTT	50.0	31.8	57.9	55.1	1135 bp	1.0	40.2