Hotspot

trnT-UGU-trnL-UAA

LSC · 48805-49859 · 1055 bp

Back to Disanthus

Pi 0
MarkerSeek score 58.80
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1055
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 AAAGCCAGCTATCGGAATCG ACCAATTTCGCCATATCCCC 50.0 50.0 58.1 58.0 1149-1150 bp 1.0 79.0
trnT-UGU-trnL-UAA_p2 CTCTAACCGCTGAGCTAAGC ACCAATTTCGCCATATCCCC 55.0 50.0 58.2 58.0 1098-1099 bp 1.0 78.8
trnT-UGU-trnL-UAA_p3 AAAGCCAGCTATCGGAATCG TCCCTACCAAGGCTCAATCA 50.0 50.0 58.1 58.0 1188-1189 bp 1.0 78.8
trnT-UGU-trnL-UAA_p4 AAAGCCAGCTATCGGAATCG CCTAGTGGGTTTCCCTACCA 50.0 55.0 58.1 58.0 1199-1200 bp 1.0 78.7
trnT-UGU-trnL-UAA_p5 CTCTAACCGCTGAGCTAAGC TCCCTACCAAGGCTCAATCA 55.0 50.0 58.2 58.0 1137-1138 bp 1.0 78.6