Hotspot

rps14-psaB

LSC · 39924-40058 · 135 bp

Back to Disanthus

Pi 0

MarkerSeek score 53.20

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 135
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	GCCTCTTCTTCTCCCTCTGA	CCAGTGGCTCTTTCCATTGT	55.0	50.0	58.1	58.1	291-292 bp	1.0	78.5
rps14-psaB_p2	TTGCCTCTTCTTCTCCCTCT	CCAGTGGCTCTTTCCATTGT	50.0	50.0	57.7	58.1	293-294 bp	1.0	76.9
rps14-psaB_p3	TCAACGACGGAACTTTGCTT	CCAGTGGCTCTTTCCATTGT	45.0	50.0	58.3	58.1	363-364 bp	1.0	76.8
rps14-psaB_p4	TTCAACGACGGAACTTTGCT	CCAGTGGCTCTTTCCATTGT	45.0	50.0	58.3	58.1	364-365 bp	1.0	76.8
rps14-psaB_p5	GCCTCTTCTTCTCCCTCTGA	GCTCTTTCCATTGTGCAAGC	55.0	50.0	58.1	58.3	285-286 bp	1.0	76.8