Hotspot

trnS-UGA-psbZ

LSC · 38106-38453 · 348 bp

Back to Disanthus

Pi 0.00287

MarkerSeek score 55.20

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 348
variable_sites: 1
pi: 0.00287
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	483 bp	1.0	78.1
trnS-UGA-psbZ_p2	CAAGACCGGAGCTATCAACC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.1	58.3	485 bp	1.0	77.6
trnS-UGA-psbZ_p3	CTATCAACCACTCGGCCATC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	472 bp	1.0	77.5
trnS-UGA-psbZ_p4	GAGCAAAGGAGAGAGAGGGA	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	546 bp	1.0	77.4
trnS-UGA-psbZ_p5	CTATCAACCACTCGGCCATC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.1	58.3	474 bp	1.0	77.1