Hotspot

matK-trnK-UUU

LSC · 3538-4238 · 701 bp

Back to Disanthus

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 701
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GAAGTCAGGTTGCCGAGATT	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.9	58.2	779 bp	1.0	77.8
matK-trnK-UUU_p2	GAAGTCAGGTTGCCGAGATT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.9	58.2	778 bp	1.0	77.8
matK-trnK-UUU_p3	GAAGTCAGGTTGCCGAGATT	GGGTTGCTAACTCAACGGTA	50.0	50.0	57.9	57.5	792 bp	1.0	75.5
matK-trnK-UUU_p4	GAAGTCAGGTTGCCGAGATT	TTCGGGTTGCTAACTCAACG	50.0	50.0	57.9	58.5	795 bp	1.0	75.2
matK-trnK-UUU_p5	ATAGGAAGTCAGGTTGCCGA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.4	58.2	782 bp	1.0	75.2